Induced mutation on Indonesian black orchid (Coelogyne pandurata Lindley) in-vitro culture by gamma irradiation

Abstract The black orchid ( Coelogyne pandurata Lindley) is one of the native Indonesian species. In its natural habitat, existence threatened by continuous forest exploitation. Coveted in global market for hybridization purposes, still considered difficult to be grown cultivation and may take years flower. Its conservation breeding carried out through a combination tissue culture mutagen application, as induced mutation increase genetic variation. This experiment aims investigate effects different levels gamma irradiation on plantlets. research design used complete random design, utilizing six (0, 5, 10, 15, 20, 25 Gy). observation was survival rate, number leaves, roots, shoots, leaf color, plantlet type. Plantlets 10 Gy have best rate new but only leaves 5 dose significantly higher than control. Plantlet growth nearly all stagnated. Root also reduced plantlets 15 above. largest morphological changes are found Gy, therefore this range recommended future orchids.